Table 1.

Biophysical properties of mutant and WT channels.

	Steady-state activation			Steady-state inactivation			Fast inactivation			Recovery from fast inactivation		Current density (0 mV)
	V1/2 (mV)	K	n	V1/2 (mV)	K	n	V1/2 (mV)	K	n	τ (ms)	n	pA/pF	n
WT	− 19.4 ± 0.8	3.1 ± 0.2	15	− 67.0 ± 0.5	5.4 ± 0.2	16	− 34.8 ± 0.7	8.2 ± 0.4	24	1.9 ± 0.2	10	− 109.5 ± 10.4	11
N641Y	− 15.0 ± 0.6***	3.4 ± 0.2	25	− 64.3 ± 1.3	6.2 ± 0.2*	20	− 33.0 ± 1.0	7.2 ± 0.4	20	1.3 ± 0.1*	8	− 183.4 ± 16.9**	16
K655R	− 16.0 ± 1.8**	3.5 ± 0.3	14	− 66.1 ± 0.8	5.2 ± 0.2	18	− 33.7 ± 1.0	8.1 ± 0.5	26	1.4 ± 0.1*	17	− 134.4 ± 12.2	10
W1150R	− 23.7 ± 1.9*	2.7 ± 0.4	12	− 65.4 ± 1.1	5.1 ± 0.2	14	− 33.5 ± 1.3	7.6 ± 0.7	17	2.1 ± 0.2	12	− 159.1 ± 15.1*	8

V_1/2, voltage midpoint activation or inactivation; K, slope factor; n, number of cells; τ, time constant.

Values represent the mean ± SEM.

*P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t-test.