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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Am J Transplant. 2019 Sep 12;20(1):298–305. doi: 10.1111/ajt.15574

Figure 2:

Figure 2:

Comparison of 2C10 binding to human, rhesus macaque, baboon, and marmoset CD40. Injections of 100, 50, 25, 12.5, 6.25 and 3.125 nM sCD40-His were applied to sensor surface containing 75 RU of 2C10R1 and association and dissociation was monitored for 300 and 600 seconds, respectively. Relative conformational integrity of all four sCD40s was verified by calibration-free concentration analysis (CFCA) (30). Representative sensorgrams with raw values in black and kinetic fit in red for each species are included in (A). The mean KD values from three independent measurements by SPR analysis of primate sCD40 and 2C10 are shown in (B). Error bars represent SEM from 3 experiments.