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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Am J Transplant. 2019 Sep 12;20(1):298–305. doi: 10.1111/ajt.15574

Figure 4:

Figure 4:

Binding kinetics of select rhesus sCD40 alanine mutants. Purified sCD40 mutants E28A (A), D69A (B), R73A (C), and E74A (D) were flowed over immobilized 2C10 and the kinetics of the interaction were characterized by SPR. Analyte injections of 80, 40, 20, 10, 5.0 and 2.5 nM were tested for each mutant. Kinetic rate constants could not be determined for the 2C10-E28A interaction, as no detectible binding was observed at concentrations as high as 500 nM (data not shown). Measurable calculated ka (E), kd (F), and KD (G) are compared to wildtype for D69A, R73A, and E74A. All mutant and wildtype protein preparations were first checked for conformation integrity by CFCA (30) and affinity measurements are corrected for active protein concentrations. Kinetic value plots represent at least three independent measurements. Error bars represent SEM.