Figure 4.

Hydrogel samples of 500 μL were placed in Trans-well membrane plates, with 500 μL of PBS in the bottom well. At each time point, 100 μL of aliquots was taken from the bottom well, whereas 100 μL of fresh PBS was added simultaneously. Aliquots were run on LCMS to quantify the concentrations of the respective antibiotics. (A) Release of minocycline, vancomycin, and gentamicin from the 0.625% alginate hydrogel over time. (B) The amount of remaining minocycline, vancomycin, gentamicin, and fluconazole in the alginate hydrogel after 7 days on deep-partial thickness porcine burn that had been inoculated with different microorganisms (Acinetobacter baumannii, Staphylococcus aureus, Pseudomonas aeruginosa). LCMS, liquid chromatography–mass spectrometry. PBS, phosphate-buffered saline.