Figure 9.

Increased circulating adiponectin (Acrp) in mice with either Gao-binge alcohol treatment or adipose-specific deletion of Atg5. A-Atg5 KO mice and matched Cre− (WT) mice were subjected to Gao-binge alcohol treatment. A: Serum adiponectin levels were measured by enzyme-linked immunosorbent assay. B: Equal amounts of serum samples were subjected to Western blot in nonheated, nondenatured conditions. Ponceau S Red staining was performed as a loading control. C: Livers were subjected to RNA extraction and real-time quantitative PCR. Data are expressed as means ± SEM (A and C). n = 6 to 8 (A). n = 4 to 5 (C). *P < 0.05 (t-test). ETOH, ethanol; HMW, high molecular weight; MMW, medium molecular weight.