Figure 5.

Infiltration of MDSCs in Colon

(A and B) Infiltration of MDSCs in colon was analyzed by flow cytometry (A) and quantification analyses of MDSC (Gr-1⁺CD11b⁺-positive cell) numbers in colon (B). Each bar represents the mean with SD (**p < 0.005; ***p < 0.001; ns, not significant). (C) Immunofluorescence analysis using anti-CD11b (red) antibody and anti-Gr-1 (green) antibody co-located in MDSCs in colons of different groups. Original magnification, 100×; scale bar = 100 μm. (D) Quantification analyses of MDSCs (Gr-1⁺CD11b⁺-positive cell) numbers in colon, with error bars representing SD (*p < 0.01; ***p < 0.005).