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. 2019 Dec 23;30:101412. doi: 10.1016/j.redox.2019.101412

Fig. 6.

Fig. 6

Nrf2(L)-KO hepatocytes show reduced activation of PPARγ in response to palmitate challenge. (A–B) The mRNA levels of Pparg1 and Pparg2. Isolated primary hepatocytes from Nrf2-LoxP and Nrf2(L)-KO mice were challenged with 0.5 mM palmitate for indicated time. *p < 0.05 vs. Veh of the same genotype; #p < 0.05 vs. Nrf2-LoxP with the same treatment. (C) Representative image of immunoblots of PPARγ1 and PPARγ2 in hepatocytes. (E and F) Relative quantitative expression of PPARγ1 and PPARγ2 in (C). (F–H) mRNA levels of Fabp4, Scd1 and Fasn. (I) TG content in hepatocytes. The cells were treated with 0.5 mM palmitate for 24 h. (J) Fatty acid uptake rate in primary hepatocytes. RFU, relative fluorescence unit. (K) OCR and (L) ECAR measured by a Seahorse XF24 Extracellular Flux Analyzer in primary hepatocytes.