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. 2019 Dec 30;6(1):e03031. doi: 10.1016/j.heliyon.2019.e03031

Figure 1.

Figure 1

Immunolocalisation of αSMA, vimentin and Cx43 in passage two CFs. Immunostaining of αSMA (red: mouse monoclonal anti-αSMA [clone IA4]), vimentin (red: mouse monoclonal anti-vimentin [clone LN-6]) and Cx43 (green: rabbit polyclonal anti-Cx43) was performed on cells grown on glass for 48 h (n= 3). All images were normalised to the individual IgG shown down the right hand side. Nuclei were counterstained with DAPI (blue). Areas surrounded by a red box are magnified within the image and the white arrow denotes punctate expression of Cx43 at the cell membrane. Images were taken at x25 magnification. Scale bar represents 100 μm.