Figure 3.

Overexpression of DNAJC14 in hH723R-Pendrin Transgenic Mouse

(A) For activation of DNAJC14 in the established hH723R-pendrin transgenic (hH723R Tg) mouse model, hH723R Tg mice were crossed with Tg mice overexpressing DNAJC14. (B) Based on western blotting of the cochlea, expression of pendrin was increased by overexpressing DNAJC14. (C) Quantification of the density of pendrin is depicted in (AU, arbitrary unit) (n = 4 in each group). ∗p < 0.05. (D) DNAJC14 expression (red) was identified in the DNAJC14 Tg(+);hH723R Tg(+);mPDS(−/−) mice, and the expression of pendrin was increased in most of the cochlear tissue (Pax2-Cre dependent), as shown by immunostaining using the Alexa 488 secondary antibody (green). DAPI (blue) was used for nuclear staining. Scale bar, 100 μm; sm, scala media; st, scala tympani; sv, scala vestibule.