Fig. 2.

Effect of ALZ003 on glioblastoma and AR expression. A. Primary mouse astrocytes were treated with ALZ003 for 48 h, and then cell viability was determined by MTT assay. Left: representative images of astrocytes. Right: cell viability. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. B, C. After treatment for 48 h, cell viability was estimated by MTT assay. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. P-value was determined by Student's t-test. D. The cultured media from U87MG and Pt#3 with or without ALZ003 treatment for 48 h was analyzed by Caspase 3/7, 8 and 9-Glo activity kits. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. (*p < 0.05, **p < 0.01). After treatment for 24 h, whole cell lysates of U87MG (E) or Pt#3 (F) were prepared and analyzed by western blotting using the anti-AR antibody. Lower panel is the quantitated results. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. (*p < 0.05, **p < 0.01).