Fig. 4.

ALZ003 increases ROS and lipid peroxidation through decreasing GPX4 expression. A. After treatment for 24 h, U87MG cells were harvested for analyzing ROS production using DHR123. The number is the mean of fluorescence representing the level of ROS. Right panel is the quantitative result. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. (*p < 0.05, **p < 0.01). Additionally, cells were analyzed for the H₂O₂ level (B), GSH/GSSG ratio (C) and GSHR activity (D). C. *p and ^#p means the significant difference between control with treatment in U87MG and Pt#3, respectively. (*p, ^#p < 0.05, **p < 0.01).