Fig. 6.

AR regulates lipid peroxidation through GPX4. A. Effect of AR knockdown on GPX4 expression. Lower panel is the quantitated results. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. P-value was determined by Student's t-test. (***p < 0.001). B. AR knockdown induces strong lipid peroxidation photographed under 100X magnification in U87MG cells. Yellow arrows indicate lipid peroxidation. The scale bar represented 0.04 mm. C. After GPX4 knockdown for 2 days followed by transfection with Flag-AR for 1 day, U87MG cells were treated with 5 μM ALZ003 for 24 h. Viability was analyzed by MTT assay. Experiments were performed three times independently, and data were expressed as mean ± s.e.m. (*p < 0.05, **p < 0.01). Follow (C), lipid peroxidation was analyzed by BODIPY staining. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)