Fig. 7.

Therapeutic effect of ALZ003 on the growth of glioblastoma in vivo. A. Luciferase-expressed U87MG cells (2 × 10⁵) were intracranially transplanted into brains of nude mice. After 10 days, luciferase activity representing tumor size was monitored by IVIS200 system on Days 10, 13, 20 and 27. Experimental mice were administrated intravenously with ALZ003 three times per week. Left: The time line for IVIS200 evaluation (black line) and drug injection (red line). B. The mouse brain was paraffin embedded and subjected to histological analysis. Left panel: Brain slides were stained by hematoxylin and eosin or stained using indicated antibodies. Stained slides were photographed by microscope with 20X magnification. The scale bar for HE staining of whole brain slice is 1 mm, and that for other IHC images is 0.5 mm. Right: The IHC signal was quantitated by 4 Scores as described in Materials and Methods. P-value is indicated and “n.s.” means “not significant”. C. The statistical analysis for luciferase activity analyzed by two-way ANOVA (***p < 0.0001). D. The comparison of survival period was performed using Log-Rank Test. P-value for each paired comparison was indicated. ALZ003 administration was terminated on Day 63. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)