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. 2019 Dec 13;20(24):6293. doi: 10.3390/ijms20246293

Figure 1.

Figure 1

Schematic diagram of the first part of experimental protocol. The experiment was divided into two parts. The first part of the experiment included following 4 groups at 2 and 24 h (n = 6 in each group) after phosphate-buffered saline (PBS) or lipopolysaccharide (LPS) spray. Control (CON) group (Group 1): Intratracheal PBS (50 μL) was sprayed into the lungs of the mice in the CON group. LPS group (Group 2): Same concentration of LPS (2 mg/kg) was sprayed into the lungs of the mice in the various experimental groups. Two of the LPS groups also received carbon dioxide by exposure to 5% carbon dioxide for 10 min (Group 3: H10L group) or 60 min (Group 4: H60L group) before the LPS spray.