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. 2019 Dec 5;20(24):6134. doi: 10.3390/ijms20246134

Figure 2.

Figure 2

TLR2 and TLR4 are expressed by PBMC, PBL, and GF cultures. Relative gene expressions of (A) TLR2 and (B) TLR4 in freshly isolated PBMCs, PBLs, and GFs (passage 5). Both PBMCs and PBLs showed higher TLR2 and TLR4 gene expression than GFs. Accordingly, PBMCs expressed more TLR2 and TLR4 than PBLs. Both genes are expressed relative to the mean of the housekeeping gene HPRT. Primer sequences are provided in Table 1. Data are presented as means + standard deviations. n = 2 buffy coats, n = 6 GF donors. Statistical significance was calculated using an ANOVA with multiple comparisons (Tukey post-hoc). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.