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. 2019 Dec 5;20(24):6134. doi: 10.3390/ijms20246134

Figure 3.

Figure 3

Monocytes play a role in the retention of PBLs and is not affected by TLR agonists. Characterization of heterogeneous cell populations: (A) after 7, (B) after 14, and (C) after 21 days. The different culture conditions are presented on x-axes (A–C), with or without monocytes, and in the presence or absence of TLR agonists. Fewer cells retain over time (note differences in values at y-axes) and in PBL conditions (without monocytes). Specification of cells from (C) (at 21 days) are shown for (D) CD56+CD3− (NK cells), (E) CD14+ cells (monocytes), (F) CD19+ (B cells), and (G) CD3+ (T cells). No significant differences were found between cell compositions of control, TLR2, TLR4, and TLR2+TLR4 conditions (calculated using an ANOVA with multiple comparisons, Tukey post-hoc). Data are presented as events per microliter, n = 2 buffy coats. Significant differences between PBMC and PBL cultures were calculated (D–G) using paired t-tests. * p < 0.05, ** p < 0.01, *** p < 0.001.