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. 2019 Dec 17;20(24):6355. doi: 10.3390/ijms20246355

Figure 2.

Figure 2

FMNL1 knockdown suppresses GBM migration and invasion. (A,B) Reverse transcription (RT)-PCR and Western blot of FMNL1 in (A) U251MG and (B) DBTRG-05MG cells transfected with control or FMNL1 siRNAs. GAPDH was used as a loading control in the Western blot assays. (C,D) FMNL1 knockdown suppressed migration and invasion of (C) U251MG and (D) DBTRG-05MG cells. Representative images of transwell migration and invasion of FMNL1 knockdown cells at 200× magnification that were quantified. Scale bar: 100 μm. Columns represent migrating or invading cells from five independent microscopic fields, with bars indicating SD. * p < 0.01 vs. cells transfected with control siRNA. FMNL1 knockdown suppressed MMP9 activity; (E) MMP9 activity in the cell-conditioned medium was evaluated using gelatin zymography.