FMNL1-mediated GBM migration and invasion depend on DIAPH1 and GOLGA2, respectively. (A,B) DIAPH1 and FMNL1 protein expression in the cells transfected with siRNA against (A) FMNL1 and (B) DIAPH1. (C) Representative images and quantification of migration and invasion of DIAPH1 knockdown cells. Original magnification 200×; scale bar: 100 μm. (D,E) Protein expression of GOLGA2 and FMNL1 in the cells transfected with siRNAs against (D) FMNL1 and (E) GOLGA2, with GAPDH as a loading control. (F) Migration and invasion of GOLGA2 knockdown cells, which were quantified. Original magnification 200×; scale bar: 100 μm. Columns represent migrating or invading cells from five independent microscopic fields, with bars indicating SD. * p < 0.01 vs. cells transfected with control siRNA.