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. 2019 Dec 17;20(24):6355. doi: 10.3390/ijms20246355

Figure 4.

Figure 4

FMNL1 overexpression induces migration but not invasion. (A) Reverse transcription (RT)-PCR (left) and Western blot (right) of FMNL1 in U87MG and KNS81 cells infected with FMNL1 or EGFP lentiviral expression vectors. (B) Representative images of transwell migration and invasion of cells overexpressing FMNL1 and EGFP shown at 200× magnification, which were quantified. Scale bar: 100 μm. Columns represent migrating or invading cells from five independent microscopic fields, with bars indicating SD. * p < 0.01 vs. cells overexpressing EGFP. (C,D) Protein expression of DIAPH1 and GOLGA2 in (C) U87MG and (D) KNS81 cells transfected with FMNL1 or EGFP lentiviral expression vectors.