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The qRT-PCR validation of mRNA expression for IGF-1, GHR, FGF1, TGFB1, TCIRG1, IL10, and ADA in liver and spleen tissue among groups of control, Zn/CuSO4, Zn/Cu–Mnt, and Zn/Cu–Thz. IGF-1: insulin like growth factor 1, GHR: growth hormone receptor, FGF1: fibroblast growth factor 1, TGFB1: transforming growth factor beta-1, TCIRG1: T-cell immune regulator 1, IL10: interleukin 10, ADA: adenosine deaminase. cDNA samples, liver/group (n = 5) and spleen/group (n = 5).
