Table 2.
Primers used for quantitative real-time PCR analysis of genes expressions.
| Gene Name | Gene Bank Accession Number | Primer Sequence (5′–3′) | Product Length (bp) | Amplification Efficiency (E Value) |
|---|---|---|---|---|
| IGF-1 | NM_001082026.1 | F: AACAAGCCCACAGGATACGG | 98 | 1.97 |
| R: TCCAGCCTCCTCAGATCACA | ||||
| GHR | NM_001082636.1 | F: ACGTGTCGAGCCAAGCTTTA | 91 | 1.91 |
| R: GTCTTCTGCTGTCCCAGACC | ||||
| FGF1 | NM_001171488.1 | F: GTGTTTGTTCCTGGAACGGC | 98 | 2.00 |
| R: CGTTTTTCTTCAGCCCCACG | ||||
| TGFB1 | XM_008249704.2 | F: TGTCCACCTGCAAGACCATC | 86 | 2.02 |
| R: CCGCAGTTTGGACAGGATCT | ||||
| TCIRG1 | AF393372.1 | F: TGTCCACCTGCAAGACCATC | 86 | 1.96 |
| R: CCGCAGTTTGGACAGGATCT | ||||
| IL10 | XM_008268045.2 | F: AGCTCTGCTATGTTGCCTGG | 94 | 1.96 |
| R: GCCTGGAAAGTGAATGCAGC | ||||
| ADA | XM_002721061.3 | F: TCAAGAAGGACCAGGCGAAC | 108 | 2.01 |
| R: CAGTAAAGCCCATGTCCCGT | ||||
| GAPDH | NM_001082253.1 | F: GTCAAGGCTGAGAACGGGAA | 95 | 2.00 |
| R: CCAGCATCACCCCACTTGAT | ||||
| β-actin | NM_001101683.1 | F: CGCAAGTACTCGGTGTGGAT | 94 | 2.02 |
| R: CCGACTCGTCATACTCCTGC |
IGF-1: insulin-like growth factor 1; GHR: growth hormone receptor; FGF1: fibroblast growth factor 1; TGFB1: transforming growth factor beta-1; TCIRG1: T-cell immune regulator 1; IL10: interleukin 10; ADA: adenosine deaminase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; β-actin: beta-actin.