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An increase in comet length after a 24 h TA treatment that is dose-dependent is observed by the comet assay (MQ × 200). This is an indication of DNA fragmentation, the longer the comet, the higher the extent of the damage in the cell. A significant increase in comet length and therefore DNA fragmentation in a non-dose dependent manner is observed. (*** p < 0.0001 using ANOVA and student t-tests).
