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. 2019 Dec 12;20(24):6280. doi: 10.3390/ijms20246280

Figure 5.

Figure 5

Farnesiferol B inhibits the activity of inflammatory cells by activating TGR5. (A) Representative images of J774 cells seeded in 3-µm pore polycarbonate membrane and stained with crystal violet for migration analysis (scale bar 100 μm) after treatment with (a) PBS, (b) LPS, (c) LPS+Farnesiferol B, or (d) PBS+Farnesiferol B. Level of mRNA for (B) TNFα, (C) Ccl2 and (D) Ccl3 with different treatments. (E) Representative immunoblotting image of nuclear protein for NF-κB p65 expression. J774 cells were treated with 100 ng/mL LPS with or without co-incubation with Farnesiferol B at 5 uM, 10 uM and 20 µM, or INT777 for 2 h. (F) NF-κB DNA binding activity. Data are means ± SD of at least three independent experiments, one-way ANOVA with Bonferroni’s test. * p < 0.05.