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Farnesiferol does not inhibit migration of inflammatory cells not expressing TGR5. Representative images of RAW 264.7 macrophages seeded in 3-µm pore polycarbonate membrane with different treatment for 2 h, and stained with crystal violet for migration assay (scale bar 100 μm). Cells were treated with (a) 100 ng/mL LPS, (b) LPS with 20 µM Farnesiferol co-incubation, and (c) LPS with a NF-κB inhibitor, benzoxathiole derivative (BOT).
