Microbicidal activity of PMMA/PDDA coatings on glass coverslips obtained from casting and drying under vacuum 50 µL of NPs dispersions in water obtained from 0.56 M MMA, 5 mg·mL−1 PDDA, 2 mM amphiphile CTAB or DODAB or lecithin during the polymerization and exhaustively dialysed thereafter. Since 5 mg·mL−1 of PDDA was used for NPs synthesis, in 0.05 mL of each dispersion used for the coatings, the PDDA amount active against microbes would be 0.25 mg. However, after dialysis, the final volume of the dialyzed dispersion increased inside the dialysis bags and diluted the dispersions by 10 to 12 (PMMA/PDDA), 10 to 19 (PMMA/PDDA/CTAB), 10 to 17 (PMMA/PDDA/DODAB), and 10 to 18 (PMMA/PDDA/lecithin); final PDDA amount in each coating was 0.210, 0.130, 0.145, and 0.140 mg, respectively.