Figure 1.

(A) FACS analysis of OSCs. Fluorescence flow cytometry analysis of OSCs after separation by immunomagnetic procedure. As shown, the Ddx4⁺ cell population was expanded after selection since the Ddx4⁺ cells were up to 75.8% of the full cortical ovarian suspension, with a remarkable fluorescence intensity, thus suggesting the high molecular expression of Ddx4 molecule. (B) In vitro differentiation to oocyte-like cells (OLCs). After 21 days of culture, small Ddx4⁺ cells differentiated in large OLCs reaching up to 75–80 μm in size, with prominent nuclei and a perinuclear accumulation of organelles. At the same time, several small Ddx4⁺ cells maintained their size in cultures together with the enlarged ones.