Figure 1.

Electrophysiological data were obtained from Xenopus oocytes containing mutant Cx43-A44V (A) and Cx43-E227D (B) hemichannels using the cell-attached patch-clamp technique. Hemichannel responses to voltage ramps between −70 and +70 mV were recorded and plotted as unitary currents. Current-voltage relations for the Cx43 mutants were essentially linear across the tested range. Transitions to a sub-conducting state (B), or the fully closed state were occasionally observed (A).