Skip to main content
. 2019 Dec 12;20(24):6265. doi: 10.3390/ijms20246265

Figure 3.

Figure 3

(A) siRNA against SNAT10 induces a significant reduction in SNAT10 mRNA expression, measured with qRT-PCR. SNAT10 expression was normalized against β-tubulin expression. Unpaired t-test with 95% confidence interval was performed, (*** p < 0.0001) (n = number of samples, SNAT10siRNA n= 2, control n = 3, scramble siRNA n = 3). (B) After Slc38a10 knockdown a significant reduction (p < 0.001) in protein translation rate, measured using OPP 488 protein translation synthesis on cells treated with OPP, incorporated into nascent peptides was visualized using fluorescent Click-iT chemistry. Unpaired t-test with 95% confidence interval was performed, (*** p < 0.0001) (n = number of cells counted in analysis, SNAT10 siRNA n = 957, control n = 2280, scramble siRNA n = 2708).