Table 1.
Details of antibodies used for fluorescent immunocytochemistry and PLA.
| Primary Antibodies | Species | Dilution | Company |
|---|---|---|---|
| Anti-SNAT10 | Rabbit | 1:100 | Innovagen |
| Anti-Synaptophysin | Mouse | 1:250 | BD Transduction lab |
| Anti-SNAP25 | Mouse | 1:500 | Millipore |
| Anti-Golgi 58k | Mouse | 1:50 | AbCam |
| Anti-KDEL | Mouse | 1:200 | Abcam |
| Anti-GFP | Chicken | 1:200 | Millipore |
| Anti-FLAG | Mouse/Rabbit | 1:200 | Invitrogen |
| Secondary antibodies | Species | Dilution | Company |
| Anti-rabbit-594 | Donkey | 1:800 | Invitrogen |
| Anti-mouse-488 | Goat | 1:800 | Invitrogen |