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. 2019 Dec 11;20(24):6255. doi: 10.3390/ijms20246255

Figure 4.

Figure 4

Percentages of viable spermatozoa with low membrane lipid disorder (M540−/YO-PRO-1−) in good freezability ejaculates (GFE; (A)) and poor freezability ejaculates (PFE; (B)) exposed to AQP inhibitors in the extender: 0.1 mmol/L, 1 mmol/L, and 10 mmol/L 1,3-propanediol (PHL) or 250 µmol/L, 500 µmol/L, and 1000 µmol/L phloretin (PHL); or not (control). Data were collected from fresh (extended) and frozen-thawed (FT) samples at 30 and 240 min post-thaw, and are shown as mean ± SEM. Different letters (a, b, c) indicate a significant difference (p < 0.05) between treatments within a given time point.