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. 2019 Dec 15;20(24):6330. doi: 10.3390/ijms20246330

Figure 8.

Figure 8

Percentages of viable spermatozoa with high calcium levels evaluated through Rhod5 (Rh5+) in relation to total viable spermatozoa (YO-PRO-1-) and fluorescence intensity of Rhod5 in viable spermatozoa with positive Rhod5-staining (C,D) throughout in vitro incubation of sperm samples in capacitation medium (negative control samples), capacitation medium plus TEA blocker (positive control samples), and capacitation medium plus PAX blocker. In experiment 1, TEA (TEA samples) or PAX (PAX samples) blockers were added to the capacitation medium at 0 min (A,C). In experiment 2, TEA (TEA acute samples) or PAX (PAX acute samples) blockers were added to the capacitation medium at 240 min of incubation (B,D). Different superscripts indicate significant differences (p < 0.05) between samples within the same time point. The arrow indicates the time at which 10 µg/mL of progesterone was added in all samples (i.e., 240 min of incubation).