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. 2019 Dec 11;20(24):6250. doi: 10.3390/ijms20246250

Table 5.

Fatty acid composition of livers after challenge test diets with substitution of FO by LO (% total fatty acids)

FHD FLD VHD VLD Diet (D) fads2(f) D × f
14:0 1.58 ± 0.09 1.66 ± 0.40 1.76 ± 0.27 1.58 ± 0.28 0.76 0.76 0.47
14:1n-7 0.01 ± 0.00 0.01 ± 0.00 0.02 ± 0.00 0.02 ± 0.00 0.14 0.69 0.52
14:1n-5 0.02 ± 0.01 0.04 ± 0.01 0.03 ± 0.00 0.03 ± 0.00 0.80 0.20 0.16
15:0 0.13 ± 0.01 0.17 ± 0.04 0.14 ± 0.02 0.14 ± 0.00 0.68 0.12 0.20
15:1n-5 0.02 ± 0.00 0.02 ± 0.00 0.02 ± 0.01 0.02 ± 0.00 0.88 0.51 0.11
16:0ISO 0.02 ± 0.00B 0.02 ± 0.00A 0.02 ± 0.00AB 0.02 ± 0.00B 0.49 0.43 0.03
16:0 15.70 ± 0.42 14.30 ± 0.96 16.02 ± 1.68 13.92 ± 1.92 0.97 0.06 0.67
16:1n-7 2.26 ± 0.17 2.49 ± 0.44 2.57 ± 0.23 2.43 ± 0.23 0.48 0.79 0.31
16:1n-5 0.11 ± 0.00B 0.13 ± 0.01A 0.12 ± 0.00B 0.14 ± 0.02A 0.24 0.04 0.38
16:2n-4 0.05 ± 0.02 0.09 ± 0.05 0.07 ± 0.02 0.08 ± 0.01 0.71 0.11 0.37
17:0 0.07 ± 0.01 0.11 ± 0.04 0.09 ± 0.02 0.10 ± 0.01 0.52 0.09 0.27
16:3n-4 0.19 ± 0.01B 0.21 ± 0.00A 0.19 ± 0.00B 0.20 ± 0.00A 0.80 0.00 0.07
16:3n-3 0.03 ± 0.00 0.04 ± 0.01 0.04 ± 0.00 0.04 ± 0.01 0.67 0.08 0.08
16:3n-1 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.11 0.50 0.19
16:4n-3 0.03 ± 0.01 0.05 ± 0.04 0.04 ± 0.02 0.05 ± 0.01 0.63 0.17 0.46
18:0 6.16 ± 0.54A 5.38 ± 0.17B 5.86 ± 0.37AB 5.40 ± 0.49B 0.57 0.03 0.52
18:1n-9 42.01 ± 0.34 40.03 ± 2.18 40.50 ± 0.48 40.05 ± 0.88 0.32 0.12 0.30
18:1n-7 2.52 ± 0.05 2.60 ± 0.14 2.54 ± 0.07 2.56 ± 0.04 0.86 0.31 0.54
18:1n-5 0.06 ± 0.01 0.08 ± 0.00 0.06 ± 0.03 0.07 ± 0.00 0.31 0.17 0.84
18:2n-9 2.04 ± 0.10 2.29 ± 0.49 2.26 ± 0.33 2.69 ± 0.88 0.35 0.30 0.78
18:2n-6 11.09 ± 0.41B 11.85 ± 0.33A 10.64 ± 0.56B 11.67 ± 0.63A 0.31 0.01 0.66
18:2n-4 0.06 ± 0.01B 0.09 ± 0.02A 0.07 ± 0.00AB 0.08 ± 0.01AB 0.85 0.01 0.26
18:3n-6 1.69 ± 0.04 2.17 ± 0.39 1.89 ± 0.46 2.49 ± 0.85 0.41 0.11 0.85
18:3n-4 0.06 ± 0.00 0.07 ± 0.01 0.07 ± 0.01 0.07 ± 0.00 0.32 0.20 0.11
18:3n-3 5.30 ± 0.24 5.67 ± 0.44 5.15 ± 0.57 5.74 ± 0.45 0.89 0.10 0.68
18:4n-3 1.21 ± 0.01 1.60 ± 0.23 1.41 ± 0.39 1.80 ± 0.53 0.36 0.09 1.00
18:4n-1 0.02 ± 0.00 0.04 ± 0.01 0.03 ± 0.00 0.04 ± 0.00 0.32 0.10 0.19
20:0 0.16 ± 0.00 0.15 ± 0.03 0.16 ± 0.02 0.14 ± 0.02 0.69 0.41 0.87
20:1n-9 0.12 ± 0.01 0.14 ± 0.02 0.12 ± 0.01 0.13 ± 0.01 0.51 0.19 0.69
20:1n-7 0.73 ± 0.05 0.71 ± 0.18 0.74 ± 0.11 0.66 ± 0.09 0.83 0.48 0.69
20:1n-5 0.07 ± 0.00B 0.08 ± 0.01AB 0.07 ± 0.01B 0.08 ± 0.00A 0.49 0.04 0.45
20:2n-9 0.75 ± 0.12AB 0.51 ± 0.12BC 0.88 ± 0.02A 0.47 ± 0.14C 0.52 0.00 0.23
20:2n-6 0.26 ± 0.02 0.25 ± 0.02 0.24 ± 0.01 0.23 ± 0.02 0.11 0.23 0.92
20:3n-9 0.00 ± 0.00 0.01 ± 0.00 0.00 ± 0.00 0.01 ± 0.00 0.71 0.12 0.48
20:3n-6 0.27 ± 0.03A 0.22 ± 0.03B 0.31 ± 0.02A 0.21 ± 0.04B 0.37 0.00 0.19
20:4n-6 0.35 ± 0.00 0.39 ± 0.05 0.35 ± 0.02 0.37 ± 0.02 0.57 0.17 0.50
20:3n-3 0.23 ± 0.02 0.22 ± 0.01 0.21 ± 0.01 0.21 ± 0.01 0.07 0.46 0.67
20:4n-3 0.30 ± 0.03 0.32 ± 0.04 0.38 ± 0.04 0.33 ± 0.01 0.07 0.39 0.12
20:5n-3 0.96 ± 0.12B 1.42 ± 0.38AB 1.21 ± 0.10AB 1.42 ± 0.16A 0.37 0.03 0.36
22:1n-1 0.17 ± 0.04 0.24 ± 0.12 0.21 ± 0.06 0.22 ± 0.02 0.87 0.35 0.53
22:1n-9 0.27 ± 0.01 0.26 ± 0.04 0.26 ± 0.04 0.24 ± 0.02 0.37 0.35 0.57
22:4n-6 0.04 ± 0.00 0.05 ± 0.01 0.05 ± 0.01 0.05 ± 0.00 0.83 0.15 0.36
22:5n-6 0.10 ± 0.00 0.12 ± 0.01 0.10 ± 0.01 0.11 ± 0.01 0.90 0.05 0.13
22:5n-3 0.39 ± 0.05B 0.61 ± 0.12A 0.49 ± 0.04AB 0.61 ± 0.09A 0.35 0.01 0.37
22:6n-3 2.37 ± 0.16B 3.09 ± 0.51AB 2.60 ± 0.18B 3.10 ± 0.38A 0.56 0.01 0.57
Σ
Saturated1 23.80 ± 0.84 21.77 ± 1.59 23.03 ± 1.78 21.29 ± 2.58 0.57 0.11 0.89
16:1n2 2.37 ± 0.17 2.62 ± 0.45 2.60 ± 0.24 2.57 ± 0.21 0.53 0.60 0.41
18:1n 44.59 ± 0.32 42.70 ± 2.04 43.53 ± 0.93 42.68 ± 0.91 0.46 0.09 0.48
20:1n 0.92 ± 0.06 0.93 ± 0.21 0.91 ± 0.12 0.87 ± 0.08 0.68 0.79 0.76
18:1/16:1 18.90 ± 1.52 16.70 ± 3.55 16.82 ± 1.72 16.70 ± 1.31 0.87 0.65 0.67

Values in the graphs are presented as mean and standard deviation (n = 3) (one pool of 5 livers per tank * 3). * S = selection, D = diet, S × D = interaction of selection and diet, p-values under 0.05 are bolded, 1 ∑ Saturated fatty acids include 14:0, 15:0, 16:0, 17:0, 18:0, and 20:0, 2 ∑ 16:1n series fatty acids include: 16:1n-5, 16:1n-7, 3 ∑ 18:1n series fatty acids include: 18:1n-9, 18:1n-7, 18:1n-5, 4 ∑ 20:1n series fatty acids include: 20:1n-9, 20:1n-7, 20:1n-5.