Table 1.

Preparative transglycosylation reactions with TfHex WT and Y470H.

Entry	Enzyme	Donor	Acceptor	Donor/Acceptor [mM] 1	Product	Yield
I	WT	pNP-GlcNAc	GlcNAc	50/300	GlcNAc-β(1-4)-GlcNAc	24%
II	WT	pNP-GalNAc	GalNAc	50/300	GalNAc- β (1-6)-GalNAc	31%
III	WT	pNP-GlcNAc	Glc	50/300	GlcNAc- β (1-1)-Glc	14%
IV	WT	pNP-GlcNAc	Gal	50/300	GlcNAc- β (1-1)-Gal	10%
V	Y470H	pNP-GlcNAc	MurNAc	50 (x2)/100	GlcNAc- β (1-X)-MurNAc	n.q. 1
VI	Y470H	pNP-GalNAc	MurNAc-OPr	50/100	GalNAc- β (1-6)-MurNAc-OPr	≈1%

¹ Not quantified. The product could not be isolated in a sufficient purity for NMR characterization.