Table 1.
Gene-specific primers for 24 selenoproteins and β-actin used in realtime quantitative reverse transcription PCR.
| Gene | Accession Number | Primer Sequence (5′→3′) |
|---|---|---|
| Glutathione peroxidase 1 (Gpx1) | AF532927 | GATGCCACTGCCCTCATGA |
| TCGAAGTTCCATGCGATGTC | ||
| Glutathione peroxidase 2 (Gpx2) | DQ898282 | AGAATGTGGCCTCGCTCTGA |
| GGCATTGCAGCTCGTTGAG | ||
| Glutathione peroxidase 3 (Gpx3) | AY368622 | TGCACTGCAGGAAGAGTTTGAA |
| CCGGTTCCTGTTTTCCAAATT | ||
| Glutathione peroxidase 4 (Gpx4) | NM_214407 | TGAGGCAAGACGGAGGTAAACT |
| TCCGTAAACCACACTCAGCATATC | ||
| Deiodinase type I (Dio1) | AY533206 | CATGGCCAAGAACCCTCACT |
| CCAGAAATACTGGGCACTGAAGA | ||
| Deiodinase type II (Dio2) | NM_001001626 | GCCACTTGACCTCCTTTCACT |
| CTGGTTCTGGTGCTTCTTCAC | ||
| Deiodinase type III (Dio3) | AY533208 | TGAAGTGGAGCTCAACAGTGATG |
| TGTCGTCAGACACGCAGATAGG | ||
| Selenophosphate synthetase 2 (Sephs2) | EF033624 | TGGCTTGATGCACACGTTTAA |
| TGCGAGTGTCCCAGAATGC | ||
| Thioredoxin reductase 1 (Txnrd1) | AF537300 | GATTTAACAAGCGGGTCATGGT |
| CAACCTACATTCACACACGTTCCT | ||
| Thioredoxin reductase 2 (Txnrd2) | NM_001168702 | CAATGCTACGACCTCCTGGT |
| GGCGAAGGGCTCACATAGTC | ||
| Thioredoxin reductase 3 (Txnrd3) | XM_005669829 | CACTTTCAGCATCCACCACAT |
| TAAATCCATCCCTTTCCTCGT | ||
| Selenoprotein H (Selh) | NM_001184948 | GAAGGGAATGGAGGAGGCTA |
| TTCACCCTCACTGGAAGCTC | ||
| Selenoprotein I (Seli) | NM_001244662 | GATGGTGTGGATGGAAAGCAA |
| GCCATGGTCAAAGAGTTCTCCTA | ||
| Selenoprotein K (Selk) | DQ372075 | CAGGAAACCCCCCTAGAAGAA |
| CTCATCCACCGGCCATTG | ||
| Selenoprotein M (Selm) | FJ968780 | CAGCTGAATCGCCTCAAAGAG |
| GAGATGTTTCATGACCAGGTTGTG | ||
| Selenoprotein N, 1 (Sepn1) | EF113595 | ACCTGGTCCCTGGTGAAAGAG |
| AGGCCAGCCAGCTTCTTGT | ||
| Selenoprotein O (Selo) | AK236851 | CTTCCGACCCCAGATGGAT |
| GGTTCGACTGTGCCAGCAT | ||
| Selenoprotein P1 (Sepp1) | EF113596 | AACCAGAAGCGCCAGACACT |
| TGCTGGCATATCTCAGTTCTCAGA | ||
| Selenoprotein S (Sels) | GU983865 | ACAGGAGGCTTTAGCAGCAG |
| CGCTGTCCCATCTTTCAATC | ||
| Selenoprotein T (Selt) | NM_001163408 | CGCTGCTCAAATTCCAGATA |
| CTCTCCTTCAATGCGGATGT | ||
| Selenoprotein V (Selv) | GQ478346 | CACTGGTCGCCAATGGATTC |
| AGTGGCCAACGGAGAAAGC | ||
| Selenoprotein W (Selw) | NM_213977 | CACCCCTGTCTCCCTGCAT |
| GAGCAGGATCACCCCAAACA | ||
| Selenoprotein X (Selx_) | EF113597 | ATCCCTAAAGGCCAAGAATCATC |
| GGCCACCAAGCAGTGTTCA | ||
| Selenoprotein 15 (Sep15) | EF178474 | ACAGCCCTGCCAAGCAGAT |
| AACAGGGAGGCTGGGTAACAC | ||
| β-actin | AY550069 | CCCAAAGCCAACCGTGAGAA |
| CCACGTACATGGCTGGGGTG |