Response of barley transcriptome at the onset and after prolonged drought stress analyzed using Agilent microarray. (A) Hierarchical clustering of the genes that were differentially expressed in barley subjected to drought. (B) Number of Differently Expressed Genes (DEGs) in response to early (onset) and prolonged drought stress (p ≤ 0.01, Fold Change (FC) ≥ 2). (C) Gene Ontology enrichment of down- and up-regulated DEGs at the onset of drought stress (p ≤ 0.05); BP–biological process, CC–cellular component, MF–molecular function. (D) The Gene Ontology enrichment of down- and up-regulated DEGs after prolonged drought stress (p ≤ 0.05); BP–biological process, CC–cellular component, MF–molecular function.