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. 2019 Dec 13;20(24):6296. doi: 10.3390/ijms20246296

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Proliferative properties of hCECs grown with iHFL or i3T3 feeder layer. (A) Human corneal epithelial cells (hCEC52) at passage P3 were grown either alone (-) or in the presence of feeder cells (either iHFL or i3T3) until they reached near 90% confluence before they were trypsinized and cultured to the next passage. Cells were passaged until they reach replicative senescence. Scale bars: 200 µm. (B) Graph representation of the growth rates calculated as the number of population doubling per day on average and determined from the hCECs cultured in panel A. (C) Total population doubling of hCECs grown either with (iHFL and i3T3) or without (-) a feeder layer for each of the three cell populations (hCEC48, hCEC52 and hCEC71).