Figure 6.

Alteration of gene expression patterns in response to the type of feeder layer used. (A) Scatter plots of log₂ of signal intensity from 60,000 different targets covering the entire human transcriptome of hCECs cultured without a feeder layer (-FL; x-axis) plotted against hCECs grown in the presence of either i3T3 (left), or iHFL (center) (+i3T3 or +1HFL; x-axis). (B) Venn diagram that depicts the number of genes differentially regulated by more than 3-fold in hCECs cultured without a feeder layer relative to hCECs grown with i3T3 (green) or iHFL (red). Genes that are commonly regulated between these two conditions are also indicated (in yellow). (C) Heatmap representation of the 55 most differentially regulated genes expressed by hCECs grown with a feeder layer (i3T3 or iHFL) relative to their levels in hCECs grown with no feeder layer.