Anti-fibrotic effects of the etanercept-secretome in an in vivo model of liver fibrosis. (A) Effects of etanercept-secretome on the serum levels of liver enzymes. Of mice with two kinds of secretome treatment (control secretome and etanercept-secretome), etanercept-secretome group showed the significantly lower AST and ALT than did control secretome group. (B) Effects of etanercept-secretome on the serum levels of pro-inflammatory cytokines (TNF-α and IL-6). Etanercept-secretome group showed the lowest serum levels of TNF-α and IL-6 of all. The values are presented as mean ± standard deviation of three independent experiments. Abbreviations: ALT, alanine transaminase; AST, aspartate transaminase; COL1A1, collagen type 1 alpha1; CS, secretome obtained from empty vector-transfected ASCs; Ct, control; ES, etanercept-secretome (secretome obtained from etanercept-synthesizing ASCs); Sal, saline injection group; TNF-α, tumor necrosis factor-α.