Figure 5.

Solution structure of CNNM4_{BAT-cNMP-Ctail} alone and in complex with PRL-1. (A) SAXS derived bead model of CNNM4_{BAT-cNMP-Ctail} in the absence of MgATP, built from the crystal structures of the isolated CNNM4_cNMP (PDB ID 6G52) and CNNM4_BAT in twisted conformation (modeled from CNNM2_BAT, PDB code 4IYS). (B) SAXS derived bead model of CNNM4_{BAT-cNMP-Ctail} in the presence of MgATP, built from the independent crystal structures of the isolated CNNM4_cNMP and CNNM4_BAT in flat conformation (modeled from CNNM2_BAT, PDB code 4P1O). The invisible disordered C-terminal tail of CNNM4_cNMP is not represented for clarity. (C) SAXS derived bead model of the complex formed by PRL-1 and CNNM4_{BAT-cNMP-Ctail} in the presence of MgATP. The model was built from the crystal structures of the homologous CNNM2_BAT complexed with both PRL-1 and ZnATP (PDB ID: 5LXQ) and CNNM4_cNMP (PDB ID: 6G52). In all structures shown, the disordered linker (green) between Bateman module and cNMP domain was modelled with CORAL [54], the putative position of the long loop within the cNMP domain is represented as a dotted line. The complementary monomer is distinguished by an asterisk. Panels to the right show the corresponding SAXS data and computed scatter curves (black) for the illustrated best-fit bead model.