. 2019 Dec 12;20(24):6279. doi: 10.3390/ijms20246279

Table 2.

Data collection and structural parameters by SAXS.

Data Collection Parameters
Beamline	B21, Diamond Light Source, Harwell (UK)
Detector	Pilatus 2M
Beam size	0.2 × 0.2 mm
Energy	12.4 keV
Sample-to-detector distance (mm)	4014
q range (A⁻¹)	0.0038–0.42
Exposure time (s)	3
Number of frames	620
Temperature (K)	293
Mode	SEC online
Structural Parameters
Protein construct	CNNM4_cNMP	CNNM4_{BAT-cNMP-Ctail}	CNNM4_{BAT-cNMP-Ctail} + MgATP	CNNM4_{BAT-cNMP-Ctail} + PRL-1	CNNM4_{BAT-cNMP-Ctail} + MgATP + PRL-1
SASBDB access code	SASDER8	SASDEQ8	SASDES8	SASDEP8	SASDEN8
Concentration range (mg/mL)	7.0	5.0	4.7	8.5	5.0
q Interval for Fourier inversion (Å⁻¹)	0.014–0.2018	0.007–0.164	0.010–0.109	0.09–0.173	0.009–0.125
R_g [from P(r)] (Å)	25.67 ± 1.32	41.62 ± 2.12	39.15 ± 1.38	47.90 ± 5.24	47.20 ± 2.55
R_g [from Guiner approximation] (Å)	25.52 ± 1.65	40.22 ± 0.54	39.23 ± 1.98	46.55 ± 0.81	44.48 ± 1.42
sR_g limits [from Guiner approx.]	0.34–1.30	0.31–1.30	0.41–1.30	0.36–1.29	0.38–1.29
D_max (Å)	96	166	143	185	183
Porod volume estimate (nm³)	102	156	149	226	216
Porod exponent	2.3	3.2	3.3	3.1	3.1
Molecular Mass (kDa)
from Porod volume (×0.58)	59	90	86	131	125
from amino acid sequence	21	48	48	68	68
Software Employed
Primary data reduction	DAWN pipeline (Diamond Light Source, UK)
Data processing	ScÅtter v3.1v
Ab initio modelling	GASBOR
Validation and averaging	DAMAVER/DAMCLUST
Computation of model intensities	CRYSOL/CORAL

q = 4πsin(θ)/λ, where 2θ is the scattering angle and λ the wavelength.