. 2019 Dec 10;20(24):6229. doi: 10.3390/ijms20246229

Table 1.

Primer sequences for RT-PCR.

Target gene	Primer Direction	Sequence (5′–3′)
AK045490	Forward:	GCATTGTATCTCGCTCCACA
AK045490	Reverse:	TGTTGCCTACCTGCTTACTGC
Alp	Forward:	GTTGCCAAGCTGGGAAGAACAC
Alp	Reverse:	CCCACCCCGCTATTCCAAAC
Ocn	Forward:	GAAGGCAACAGTCGATTCACC
Ocn	Reverse:	GACTGTCTTGCCCCAAGTTCC
Col Ⅰα1	Forward:	GAAGGCAACAGTCGATTCACC
Col Ⅰα1	Reverse:	GACTGTCTTGCCCCAAGTTCC
Tcf1	Forward:	CAGAATCCACAGATACAGCA
Tcf1	Reverse:	CAGCCTTTGAAATCTTCATC
Let1	Forward:	GATCCCCTTCAAGGACGAAG
Let1	Reverse:	GGCTTGTCTGACCACCTCAT
Runx2	Forward:	CGCCCCTCCCTGAACTCT
Runx2	Reverse:	TGCCTGCCTGGGATCTGTA
Gapdh	Forward:	TGCACCACCAACTGCTTAG
Gapdh	Reverse:	GGATGCAGGGATGATGTTC

Alp: alkaline phosphatase; Ocn: Osteocalcin; Col Iα1: collagen type I α1; Tcf1: transcription factor 7; Lef1: lymphoid enhancer binding factor 1; Runx2: runt-related transcription factor 2; Gapdh: glyceraldehyde 3-phosphate dehydrogenase.