Skip to main content
. 2019 Dec 11;20(24):6238. doi: 10.3390/ijms20246238

Figure 1.

Figure 1

Arginine-rich dipeptide repeat proteins (DRPs) dose-dependently decrease WST-1 absorbance more potently in neuronal cell types. Dose-dependent, cell type-specific reduction of metabolic activity was observed in Chinese hamster ovary (CHO) and mouse spinal cord x neuroblastoma hybrid (NSC-34) cells treated with 30 and 3 μM doses of poly-glycine-arginine (GR15) (a) and poly-proline-arginine (PR15) (b). These results were not observed in CHO and NSC-34 cells treated with 30 and 3 μM doses of non-arginine-rich DRPs poly-glycine-proline (GP15) (c) and poly-proline-alanine (PA15) (d), with the exception of 30 μM GP15 on NSC-34 cells. ** denotes p < 0.01, *** denotes p < 0.001, and **** denotes p < 0.0001 (comparing DRP-treated groups for each cell type to corresponding Dimethyl Sulfoxide (DMSO) controls by one-way ANOVA analysis of variance followed by Dunnett’s test). Data are presented as mean values ± standard deviations (error bars).