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. 2019 Dec 10;20(24):6233. doi: 10.3390/ijms20246233

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EVs characterization and quantification. Plasma EVs size distribution was determined using electron microscopy. EVs were also characterized using flow cytometry, and quantified using a commercial kit. Panel (A) shows the size distribution of EVs. Panel (B) shows flow cytometry analysis of purified plasma EVs following specific isolation with magnetic beads stained with anti-CD9, CD63, and CD81. Panel (C) The Exo-Flow magnetic stand for EVs separation and FACS analysis shows the presence of EVs (positive, blue color) and absence of EVs (negative, red color); beads are displayed on the FACS plot (n = 6).