. 2019 Dec 16;20(24):6339. doi: 10.3390/ijms20246339

Table 1.

Samples of bovine oviductal extracellular vesicles (oEVs) used for proton nuclear magnetic resonance spectroscopy (NMR) analysis.

Stage of the Cycle	No. Animals ¹	Side Relative to Ovulation	Number of OF Pools	Volume (µL) of OF before oEV Isolation	Protein Concentration (mg mL⁻¹)
Post-ovulatory	54	Ipsilateral	4	320–420	10.4–13.6
Post-ovulatory	54	Contralateral	4	288–385	9.7–17.4
Mid-luteal	40	Ipsilateral	3	355–400	11.4–12.6
Mid-luteal	40	Contralateral	3	305–410	11.1–13.5
Late-luteal	34	Ipsilateral	3	315–470	9.4–10.3
Late-luteal	34	Contralateral	3	350–470	13.1–15.9
Pre-ovulatory	40	Ipsilateral	3	450–495	10.0–15.2
Pre-ovulatory	40	Contralateral	3	500–580	12.0–13.4

¹ Number of animals used to obtain 3–4 pools of oviductal fluid (OF) per stage × side with a minimum volume of 280 µL/pool that was used for oEV isolation.