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. 2019 Dec 16;9(12):1154. doi: 10.3390/ani9121154

Table 6.

Retention (%) in vitamin E activity in different storage periods for vitamin and vitamin/trace mineral (VTM) premixes 1.

Items Choline Cu and Zn Months (Time) SEM p-Time
1 2 3 6 12
Vitamin premix 1 - - 99.01 97.57 96.98 95.49 95.96 1.76 0.685
Vitamin premix 2 + - 97.38 97.24 96.77 99.48 95.70 1.08 0.185
SEM 0.87 1.17 0.55 2.68 1.06
p-Choline 0.197 0.851 0.797 0.318 0.870
VTM premix 1 - Low 98.70 a 97.04 ab 96.94 ab 97.74 ab 93.93 b 0.97 <0.01
VTM premix 2 + Low 99.61 a 96.57 ab 96.70 ab 97.44 ab 93.32 b 1.25 0.028
VTM premix 3 - High 99.18 a 96.99 ab 96.60 ab 96.52 ab 94.08 b 0.76 <0.01
VTM premix 4 + High 99.29 a 98.20 ab 96.38 bc 97.55 ab 93.82 c 0.63 <0.01
SEM 0.32 0.65 1.02 1.63 0.57
Main effects
Choline - 98.94 97.01 96.29 97.13 94.00
+ 99.45 97.38 96.54 97.50 93.57
Cu and Zn Low 99.16 96.80 96.34 97.59 93.63
High 99.23 97.59 96.49 97.03 93.95
p-value
Choline 0.130 0.582 0.748 0.835 0.455
Cu and Zn 0.822 0.241 0.851 0.750 0.577
Choline x Cu and Zn 0.229 0.214 0.556 0.705 0.760

1 Vitamin premix 1 contained no choline chloride, while Vitamin premix 2 contained 160,000 mg/kg of choline chloride. VTM premix 1 contained 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO, and in addition, VTM premix 2 contained 40,000 mg/kg of choline chloride, 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO. VTM premix 3 contained 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. VTM premix 4 contained 40,000 mg/kg of choline chloride, 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. a, b, c Means in a row, with different superscripts, are different (p < 0.05).