Table 6.
Items | Choline | Cu and Zn | Months (Time) | SEM | p-Time | ||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 6 | 12 | |||||
Vitamin premix 1 | - | - | 99.01 | 97.57 | 96.98 | 95.49 | 95.96 | 1.76 | 0.685 |
Vitamin premix 2 | + | - | 97.38 | 97.24 | 96.77 | 99.48 | 95.70 | 1.08 | 0.185 |
SEM | 0.87 | 1.17 | 0.55 | 2.68 | 1.06 | ||||
p-Choline | 0.197 | 0.851 | 0.797 | 0.318 | 0.870 | ||||
VTM premix 1 | - | Low | 98.70 a | 97.04 ab | 96.94 ab | 97.74 ab | 93.93 b | 0.97 | <0.01 |
VTM premix 2 | + | Low | 99.61 a | 96.57 ab | 96.70 ab | 97.44 ab | 93.32 b | 1.25 | 0.028 |
VTM premix 3 | - | High | 99.18 a | 96.99 ab | 96.60 ab | 96.52 ab | 94.08 b | 0.76 | <0.01 |
VTM premix 4 | + | High | 99.29 a | 98.20 ab | 96.38 bc | 97.55 ab | 93.82 c | 0.63 | <0.01 |
SEM | 0.32 | 0.65 | 1.02 | 1.63 | 0.57 | ||||
Main effects | |||||||||
Choline | - | 98.94 | 97.01 | 96.29 | 97.13 | 94.00 | |||
+ | 99.45 | 97.38 | 96.54 | 97.50 | 93.57 | ||||
Cu and Zn | Low | 99.16 | 96.80 | 96.34 | 97.59 | 93.63 | |||
High | 99.23 | 97.59 | 96.49 | 97.03 | 93.95 | ||||
p-value | |||||||||
Choline | 0.130 | 0.582 | 0.748 | 0.835 | 0.455 | ||||
Cu and Zn | 0.822 | 0.241 | 0.851 | 0.750 | 0.577 | ||||
Choline x Cu and Zn | 0.229 | 0.214 | 0.556 | 0.705 | 0.760 |
1 Vitamin premix 1 contained no choline chloride, while Vitamin premix 2 contained 160,000 mg/kg of choline chloride. VTM premix 1 contained 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO, and in addition, VTM premix 2 contained 40,000 mg/kg of choline chloride, 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO. VTM premix 3 contained 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. VTM premix 4 contained 40,000 mg/kg of choline chloride, 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. a, b, c Means in a row, with different superscripts, are different (p < 0.05).