Table 7.
Items | Choline | Cu and Zn | Months (Time) | SEM | p-Time | ||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 6 | 12 | |||||
Vitamin premix 1 | - | - | 98.66 | 97.82 | 96.79 | 95.78 | 95.33 | 1.17 | 0.221 |
Vitamin premix 2 | + | - | 97.26 a | 96.73 a | 95.40 a | 97.30 a | 92.17 b | 0.69 | <0.01 |
SEM | 0.88 | 0.56 | 0.86 | 0.99 | 1.53 | ||||
p-Choline | 0.288 | 0.176 | 0.057 | 0.304 | 0.018 | ||||
VTM premix 1 | - | Low | 99.01 a | 98.00 ab | 97.13 ab | 93.68 bc | 90.86 c | 1.18 | <0.01 |
VTM premix 2 | + | Low | 98.96 a | 97.21 ab | 97.77 a | 94.96 a | 90.26 b | 1.06 | <0.01 |
VTM premix 3 | - | High | 98.86 a | 98.36 a | 96.79 ab | 93.69 ab | 91.01 b | 1.47 | <0.01 |
VTM premix 4 | + | High | 98.84 a | 98.24 a | 97.82 ab | 93.31 bc | 91.38 c | 1.15 | <0.01 |
SEM | 0.36 | 1.67 | 1.80 | 1.08 | 0.43 | ||||
Main effects | |||||||||
Choline | - | 98.93 | 98.18 | 96.96 | 93.68 | 90.94 | |||
+ | 98.90 | 97.72 | 97.79 | 94.14 | 90.82 | ||||
Cu and Zn | Low | 98.98 | 97.60 | 97.45 | 94.32 | 90.56 | |||
High | 98.85 | 98.30 | 97.31 | 93.50 | 91.19 | ||||
p-value | |||||||||
Choline | 0.928 | 0.787 | 0.649 | 0.679 | 0.789 | ||||
Cu and Zn | 0.714 | 0.682 | 0.938 | 0.458 | 0.157 | ||||
Choline x Cu and Zn | 0.971 | 0.844 | 0.916 | 0.451 | 0.273 |
1 Vitamin premix 1 contained no choline chloride, whereas Vitamin premix 2 contained 160,000 mg/kg of choline chloride. VTM premix 1 contained 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO, and VTM premix 2 contained 40,000 mg/kg of choline chloride, 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO. VTM premix 3 contained 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. VTM premix 4 contained 40,000 mg/kg of choline chloride, 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. a, b, c Means in a row, with different superscripts, are different (p < 0.05).