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. 2019 Dec 16;9(12):1154. doi: 10.3390/ani9121154

Table 7.

Retention (%) in vitamin B6 activity in different storage periods for vitamin and vitamin/trace mineral (VTM) premixes 1.

Items Choline Cu and Zn Months (Time) SEM p-Time
1 2 3 6 12
Vitamin premix 1 - - 98.66 97.82 96.79 95.78 95.33 1.17 0.221
Vitamin premix 2 + - 97.26 a 96.73 a 95.40 a 97.30 a 92.17 b 0.69 <0.01
SEM 0.88 0.56 0.86 0.99 1.53
p-Choline 0.288 0.176 0.057 0.304 0.018
VTM premix 1 - Low 99.01 a 98.00 ab 97.13 ab 93.68 bc 90.86 c 1.18 <0.01
VTM premix 2 + Low 98.96 a 97.21 ab 97.77 a 94.96 a 90.26 b 1.06 <0.01
VTM premix 3 - High 98.86 a 98.36 a 96.79 ab 93.69 ab 91.01 b 1.47 <0.01
VTM premix 4 + High 98.84 a 98.24 a 97.82 ab 93.31 bc 91.38 c 1.15 <0.01
SEM 0.36 1.67 1.80 1.08 0.43
Main effects
Choline - 98.93 98.18 96.96 93.68 90.94
+ 98.90 97.72 97.79 94.14 90.82
Cu and Zn Low 98.98 97.60 97.45 94.32 90.56
High 98.85 98.30 97.31 93.50 91.19
p-value
Choline 0.928 0.787 0.649 0.679 0.789
Cu and Zn 0.714 0.682 0.938 0.458 0.157
Choline x Cu and Zn 0.971 0.844 0.916 0.451 0.273

1 Vitamin premix 1 contained no choline chloride, whereas Vitamin premix 2 contained 160,000 mg/kg of choline chloride. VTM premix 1 contained 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO, and VTM premix 2 contained 40,000 mg/kg of choline chloride, 500 mg/kg of CuSO4 and 8000 mg/kg of ZnO. VTM premix 3 contained 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. VTM premix 4 contained 40,000 mg/kg of choline chloride, 20,000 mg/kg of CuSO4 and 225,000 mg/kg of ZnO. a, b, c Means in a row, with different superscripts, are different (p < 0.05).