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. 2019 Dec 16;20(24):6335. doi: 10.3390/ijms20246335

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SMT did not reduce iNOS expressions but inhibited NO production and caspase activities in vivo. (A) SMT had no effect on the mRNA transcription of iNOS by qRT-PCR (n = 3). (B) SMT had no effect on the protein expression of iNOS. The expression of iNOS was detected by Western blot, and quantitated by ImageJ. (C) The NO content in muscle was significantly decreased in GCRV+SMT group VS. GCRV group. Quantification of NO production was assayed by NO Content Assay Kit (n = 3). (D) The activities of caspases were significantly decreased in GCRV+SMT group VS. GCRV group. The activity of caspase-3 and 9 were assayed by Caspase Activity Assay Kits (n = 3). (** p < 0.01, *** p < 0.001).