Figure 5.

Hemorrhage status in individuals. (A) Hemorrhage was decreased when inhibiting the function of iNOS by SMT. Viewed from the surface of the rare minnow in different groups. The fading of the glossiness of the body surface and severe subcutaneous hemorrhage could be observed in the GCRV group. The slight fading of the glossiness of the body surface and minor subcutaneous hemorrhage in the GCRV+SMT group. The blank group has a normal body color and no subcutaneous hemorrhage. (B) H&E-stained sections show the infiltration of blood cells in muscle in different treatments. The black arrows show the blood cells (scale 50 μm). (C) The H&E-stained sections from (B) were analyzed with ImageJ image-analysis software to calculate the percent of blood cell infiltration. Black dots show the blood cells in muscle tissue. (D) Rare minnow died from the fifth day after GCRV infection and all died on the seventh day.