Figure 3.

Protein fractions obtained by Ni²⁺-chelating chromatography were separated by SDS–PAGE on 12% polyacrylamide gel and stained with Coomassie blue. Lane 1, insoluble fraction of IPTG-induced cell lysate (6 μg); lane 2, soluble fraction of IPTG-induced cell lysate (28 μg); lane 3, first flow-through fraction (19 µg), lane 4, proteins eluted with 50 mM imidazole (13 µg), lane 5, first fraction of proteins eluted with 100 mM imidazole (2 µg), lane 6, second fraction of proteins eluted with 100 mM imidazole (3 µg), lane 7, third fraction of proteins eluted with 100 mM imidazole (2 μg); lane 8, first fraction of proteins eluted with 250 mM imidazole (5 μg); lane 9, second fraction of proteins eluted with 250 mM imidazole (1 μg); and lane 10, molecular mass markers.