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Absorption spectra of hFADS6 and D238A-hFADS6 purified to homogeneity. The spectra of WT hFADS6 (8.6 μM, grey dotted line) and of D238A-hFADS6 (9.7 µM, black line) were recorded in 40 mM HEPES/Na, 5 mM β-mercaptoethanol, pH 7.4. The protein concentration was measured as indicated in [21].
